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Product Name
RNPS1 antibody
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Description
RNPS1 Rabbit Polyclonal antibody. Positive IF detected in HEK-293 cells. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, human brain tissue, mouse brain tissue, mouse kidney tissue, Raji cells. Observed molecular weight by Western-blot: 55-60kd
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
E5.1 antibody; LDC2 antibody; RNPS1 antibody; SR related protein LDC2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RNPS1 recombinant protein (Accession Number: NM_080594). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:114772(RNPS1 antibody) at dilution of 1:1000
IP Result of anti-RNPS1 (IP:Catalog No:114772, 4ug; Detection:Catalog No:114772 1:800) with HEK-293 cells lysate 3200ug.
Immunofluorescent analysis of HEK-293 cells using Catalog No:114772(RNPS1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The splicing process of mRNA must be highly precise to carry the correct genetic information to the cytoplasm as templates for translation. And this process involves more than 50 proteins in the splicing complex. RNPS1 is a versatile factor that regulates alternative splicing of a number of pre-mRNAs. It participates in both constitutive splicing and in distinctive modulation of alternative splicing in a substrate dependent manner, and involved in UPF2-dependent nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Its ser-53 phosphorylation site is important for splicing and translation stimulation activity in vitro
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