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Product Name
RNH1 antibody
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Description
RNH1 Rabbit Polyclonal antibody. Positive IP detected in mouse liver tissue. Positive WB detected in L02 cells, human brain tissue, human heart tissue, human liver tissue, mouse liver tissue. Positive IHC detected in human liver tissue, human ovary tumor tissue. Positive IF detected in MCF-7 cells. Positive FC detected in MCF-7 cells. Observed molecular weight by Western-blot: 45-50 kDa
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Tested applications
ELISA, WB, IHC, IF, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Placental RNase inhibitor antibody; PRI antibody; RAI antibody; Ribonuclease inhibitor antibody; RNH antibody; RNH1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RNH1 recombinant protein (Accession Number: NM_203388). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:114767(RNH1 antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human liver using Catalog No:114767(RNH1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:114767(RNH1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using RNH1 antibody Catalog No:114767 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-RNH1 (IP:Catalog No:114767, 4ug; Detection:Catalog No:114767 1:500) with mouse liver tissue lysate 6400ug.
1X10^6 MCF-7 cells were stained with 0.2ug RNH1 antibody (Catalog No:114767, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
Ribonuclease/ angiogenin inhibitor (RNH, synonyms: RAI, MGC4569, MGC18200, MGC54054) is a protein that binds tightly to ribonucleases in cells and may be essential in the control of mRNA degradation and gene expression. The human RNH gene has been regionally localized to chromosome band 11p15 by in situ hybridization.
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References
- Yamasaki S, Ivanov P, Hu GF, Anderson P. Angiogenin cleaves tRNA and promotes stress-induced translational repression. The Journal of cell biology. 185(1):35-42. 2009.
- Stephens AN, Hannan NJ, Rainczuk A. Post-translational modifications and protein-specific isoforms in endometriosis revealed by 2D DIGE. Journal of proteome research. 9(5):2438-49. 2010.
- Kim YJ, Park SJ, Choi EY. PTEN modulates miR-21 processing via RNA-regulatory protein RNH1. PloS one. 6(12):e28308. 2011.
- Saikia M, Krokowski D, Guan BJ. Genome-wide identification and quantitative analysis of cleaved tRNA fragments induced by cellular stress. The Journal of biological chemistry. 287(51):42708-25. 2012.
- Honda S, Loher P, Shigematsu M. Sex hormone-dependent tRNA halves enhance cell proliferation in breast and prostate cancers. Proceedings of the National Academy of Sciences of the United States of America. 112(29):E3816-25. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"