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Product Name
RNF7 Polyclonal Antibody
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Description
Polyclonal antibody to RNF7
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
RNF7 antibody; CKBBP1 antibody; ROC2 antibody; SAG antibody; RING-box protein 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-113 of human RNF7 (NP_055060.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:20 - 1:50 -
Validations
Immunofluorescence - RNF7 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using RNF7 antibody . Blue: DAPI for nuclear staining.
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Background
Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets (By similarity). Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"