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Product Name
RNF20 antibody
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Description
RNF20 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A431 cells, HEK-293 cells, Jurkat cells, MCF7 cells, MCF-7 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue. Positive IP detected in HeLa cells. Positive IF detected in HepG2 cells, Hela cells. Observed molecular weight by Western-blot: 114 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BRE1 antibody; BRE1 A antibody; BRE1A antibody; FLJ11189 antibody; FLJ20382 antibody; hBRE1 antibody; KIAA2779 antibody; ring finger protein 20 antibody; RNF20 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RNF20 recombinant protein (Accession Number: NM_019592). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114751(RNF20 antibody) at dilution of 1:500
IP Result of anti-RNF20 (IP:Catalog No:114751, 3ug; Detection:Catalog No:114751 1:500) with HeLa cells lysate 2000ug.
Immunofluorescent analysis of HepG2 cells, using RNF20 antibody Catalog No:114751 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
RNF20 represents ring finger protein 20
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"