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Product Name
RNF181 antibody
- Documents
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Description
RNF181 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human kidney tissue, human heart tissue. Positive IP detected in Jurkat cells. Positive WB detected in Jurkat cells, L02 cells. Observed molecular weight by Western-blot: 18kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HSPC238 antibody; ring finger protein 181 antibody; RN181 antibody; RNF181 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RNF181 recombinant protein (Accession Number: NM_016494). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:114749(RNF181 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human kidney using Catalog No:114749(RNF181 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:114749(RNF181 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using RNF181 antibody Catalog No:114749 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-RNF181 (IP:Catalog No:114749, 3ug; Detection:Catalog No:114749 1:500) with Jurkat cells lysate 1200ug.
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Background
RNF181 represents ring finger protein 181
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References
- Tan JY, Chen JL, Huang X, Yuan CL. Screening and verification of proteins that interact with HSPC238. Oncology reports. 34(6):3097-103. 2015.
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