RNASEH2B Polyclonal Antibody

Cat.#: 164432

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Product Information

  • Product Name
    RNASEH2B Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to RNASEH2B
  • Tested applications
    WB
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    RNASEH2B antibody; AGS2 antibody; DLEU8 antibody; ribonuclease H2 subunit B antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human RNASEH2B (NP_078846.2).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - RNASEH2B Polyclonal Antibody

    Western blot - RNASEH2B Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using RNASEH2B antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.

    Western blot - RNASEH2B Polyclonal Antibody

    Western blot - RNASEH2B Polyclonal Antibody

    Western blot analysis of extracts of mouse thymus, using RNASEH2B antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

  • Background
    Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"