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Product Name
RNASEH2A antibody
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Description
RNASEH2A Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue, human spleen tissue. Positive WB detected in human liver tissue, HeLa cells. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 33 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
AGS4 antibody; JUNB antibody; Ribonuclease H2 subunit A antibody; ribonuclease H2 antibody; subunit A antibody; Ribonuclease HI large subunit antibody; Ribonuclease HI subunit A antibody; RNase H(35) antibody; RNase H2 subunit A antibody; RNase HI large subunit antibody; RNASEH2A antibody; RNASEHI antibody; RNHIA antibody; RNHL antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RNASEH2A recombinant protein (Accession Number: NM_006397). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:114729(RNASEH2A antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human testis using Catalog No:114729(RNASEH2A antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:114729(RNASEH2A antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-RNASEH2A (IP:Catalog No:114729, 4ug; Detection:Catalog No:114729 1:500) with mouse liver tissue lysate 4000ug.
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Background
RNASEH2A(Ribonuclease H2 subunit A) is also named as RNASEHI, RNHIA and belongs to the RNase HII family and Eukaryotic subfamily. It is involved in the removal of the initiator RNA primers of Okazaki fragments, an essential step during chromosomal DNA replication of the lagging strand(PMID:9482870). It is an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Defects in RNASEH2A are the cause of Aicardi-Goutieres syndrome type 4 (AGS4).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"