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Product Name
RNASEH1 antibody
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Description
RNASEH1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, human brain tissue. Observed molecular weight by Western-blot: 32 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
H1RNA antibody; Ribonuclease H type II antibody; ribonuclease H1 antibody; RNase H1 antibody; RNASEH1 antibody; RNH1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RNASEH1 recombinant protein (Accession Number: NM_002936). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114728(RNASEH1 antibody) at dilution of 1:500
IP Result of anti-RNASEH1 (IP:Catalog No:114728, 3ug; Detection:Catalog No:114728 1:500) with HeLa cells lysate 1200ug.
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Background
Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].
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References
- Bhatia V, Barroso SI, García-Rubio ML, Tumini E, Herrera-Moyano E, Aguilera A. BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2. Nature. 511(7509):362-5. 2014.
- Herrera-Moyano E, Mergui X, García-Rubio ML, Barroso S, Aguilera A. The yeast and human FACT chromatin-reorganizing complexes solve R-loop-mediated transcription-replication conflicts. Genes & development. 28(7):735-48. 2014.
- Hori S, Yamamoto T, Obika S. XRN2 is required for the degradation of target RNAs by RNase H1-dependent antisense oligonucleotides. Biochemical and biophysical research communications. 464(2):506-11. 2015.
- Hill SJ, Rolland T, Adelmant G. Systematic screening reveals a role for BRCA1 in the response to transcription-associated DNA damage. Genes & development. 28(17):1957-75. 2014.
- García-Rubio ML, Pérez-Calero C, Barroso SI. The Fanconi Anemia Pathway Protects Genome Integrity from R-loops. PLoS genetics. 11(11):e1005674. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"