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Product Name
RIP3 antibody
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Description
RIP3 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, NIH/3T3 cells. Positive IP detected in SW 1990 cells. Observed molecular weight by Western-blot: 57kd
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Tested applications
ELISA, IP, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
Receptor interacting protein 3 antibody; RIP 3 antibody; RIP like protein kinase 3 antibody; RIP3 antibody; RIPK3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RIP3 recombinant protein (Accession Number: NM_006871). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:114714(RIPK3 Antibody) at dilution of 1:300
IP Result of anti-RIPK3 (IP:Catalog No:114714, 3ug; Detection:Catalog No:114714 1:300) with SW 1990 cells lysate 3000ug.
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Background
RIPK3,also named as RIP3, a Ser/Thr kinase of RIP (Receptor Interacting Protein) family, is recruited to the TNFR1 signaling complex through RIP and has been shown to mediate apoptosis induction and NF-κB activation. RIPK3 is a nucleocytoplasmic shuttling protein and its unconventional nuclear localization signal (NLS, 442–472 aa) is sufficient to trigger apoptosis in the nucleus(PMID:18533105). It has 3 isoforms produced by alternative splicing. RIPK3 might form a homodimer within cells, and its apoptotic activity may not be required for this dimerization(PMID:18533105).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"