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Product Name
RIOK3 antibody
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Description
RIOK3 Rabbit Polyclonal antibody. Positive IP detected in mouse testis tissue. Positive WB detected in mouse testis tissue, MCF7 cells. Positive IF detected in MCF-7 cells. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 60 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp779L1370 antibody; RIO kinase 3 antibody; RIO kinase 3 (yeast) antibody; RIOK3 antibody; SUDD antibody; sudD homolog antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RIOK3 recombinant protein (Accession Number: NM_003831). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:114713(RIOK3 antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114713(RIOK3 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114713(RIOK3 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-RIOK3 (IP:Catalog No:114713, 4ug; Detection:Catalog No:114713 1:600) with mouse testis tissue lysate 4000ug.
Immunofluorescent analysis of MCF-7 cells using Catalog No:114713(RIOK3 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
RIOK3(Serine/threonine-protein kinase RIO3) is initially characterized as a homolog of Aspergillus nidulans sudD and showed down-regulation at the invasive front of malignant melanomas.On Western blots, human RIOK3 migrated as a 60-kD protein. Northern blot analysis indicated that the approximately 4-kb RIOK3 mRNA is expressed widely in human tissues, with additional smaller transcripts present in testis.
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References
- Zhang L, Flygare J, Wong P, Lim B, Lodish HF. miR-191 regulates mouse erythroblast enucleation by down-regulating Riok3 and Mxi1. Genes & development. 25(2):119-24. 2011.
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