RI-muEIP1 (Retroinverso muEIP1) peptide

RI-muEIP1 (Retroinverso muEIP1) peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-GRRRQRRKKRGGTFLGPPL-OH (All D-amino acids)

H-D-Gly-D-Arg-D-Arg-D-Arg-D-Gln-D-Arg-D-Arg-D-Lys-D-Lys-D-Arg-D-Gly-D-Gly-D-Thr-D-Phe-D-Leu-D-Gly-D-Pro-D-Pro-D-Leu-OH

19

95.2%

C₉₆H₁₇₀N₄₀O₂₂

2236.62

Synthetic

Powder

The Retroinverso(RI) ERG inhibitory peptides(EIPs) suppress tumor growth, intravasation, and metastasis in vivo. Dr. Wang first investigated the effects of EIPs on ERG protein degradation in the in vivo model at early time points. The VCaP-xenografted mice were treated with 25 mg/kg RI-EIP1 or RI-muEIP1 daily for 5 days. The tumors were then collected at day 7, and immunoblot analysis showed that RI-EIP1 was able to degrade ERG protein, while control peptide had no effect.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Wang et al., Development of Peptidomimetic Inhibitors of the ERG Gene Fusion Product in Prostate Cancer, Cancer Cell (2017)

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.