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Product Name
RHPN2 antibody
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Description
RHPN2 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in COLO 320 cells, HepG2 cells. Observed molecular weight by Western-blot: 77 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
76 kDa RhoB effector protein antibody; GTP Rho binding protein 2 antibody; p76RBE antibody; RhoBP antibody; Rhophilin 2 antibody; RHPN2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RHPN2 recombinant protein (Accession Number: NM_033103). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:20-1:200
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Validations
COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:114667(RHPN2 Antibody) at dilution of 1:600
Immunofluorescent analysis of HepG2 cells using Catalog No:114667(RHPN2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
RHPN2 binds specifically to GTP-Rho. It may function in a Rho pathway to limit stress fiber formation and/or increase the turnover of F-actin structures in the absence of high levels of RhoA activity. The MW of this protein is 77 kDa, and this antibody specially recognises the 77 kDa protein.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"