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Product Name
RHOU Polyclonal Antibody
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Description
Polyclonal antibody to RHOU
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Tested applications
WB
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Species reactivity
Human
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Alternative names
RHOU antibody; ARHU antibody; CDC42L1 antibody; G28K antibody; WRCH1 antibody; hG28K antibody; ras homolog family member U antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human RHOU.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - RHOU Polyclonal Antibody
Western blot analysis of extracts of U-87MG cells,using RHOU antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.
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Background
Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP-bound. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"