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Product Name
RHOT1 Polyclonal Antibody
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Description
Polyclonal antibody to RHOT1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
RHOT1 antibody; ARHT1 antibody; MIRO-1 antibody; MIRO1 antibody; mitochondrial Rho GTPase 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 310-590 of human RHOT1 (NP_060777.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - RHOT1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using RHOT1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Immunohistochemistry - RHOT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using RHOT1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - RHOT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human esophagus using RHOT1 antibody at dilution of 1:100 (40x lens).
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Background
Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"