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RHEB antibody

Cat.#: 114656

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Product Information

  • Product Name
    RHEB antibody
  • Documents
  • Description
    RHEB Rabbit Polyclonal antibody. Positive WB detected in human brain tissue, DU 145 cells, fetal human brain tissue, HeLa cells. Positive IHC detected in mouse brain tissue. Observed molecular weight by Western-blot: 20 kDa
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    GTP binding protein Rheb antibody; Ras homolog enriched in brain antibody; RHEB antibody; RHEB2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of RHEB recombinant protein (Accession Number: NM_005614). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114656(RHEB antibody) at dilution of 1:500

    human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114656(RHEB antibody) at dilution of 1:500

    Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:114656(RHEB Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:114656(RHEB Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:114656(RHEB Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:114656(RHEB Antibody) at dilution of 1:50 (under 40x lens)

  • Background
    Rheb is a member of the Ras superfamily small GTPases that plays important roles in the regulation of protein synthesis and growth in response to nutrient and growth factors. It is well known to bind and activate mTOR, a serine/threonine kinase which augments protein synthesis. Rheb has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity.
  • References
    • Shimizu N, Yoshikawa N, Ito N. Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle. Cell metabolism. 13(2):170-82. 2011.
    • Zheng M, Zang S, Xie L. Rheb phosphorylation is involved in p38-regulated/activated protein kinase-mediated tumor suppression in liver cancer. Oncology letters. 10(3):1655-1661. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"