RFC5 antibody

Cat.#: 114626

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Product Information

  • Product Name
    RFC5 antibody
  • Documents
  • Description
    RFC5 Rabbit Polyclonal antibody. Positive WB detected in Transfected HEK-293 cells.
  • Tested applications
    ELISA, WB
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    A1 36 kDa subunit antibody; Activator 1 36 kDa subunit antibody; Activator 1 subunit 5 antibody; Replication factor C subunit 5 antibody; RF C 36 kDa subunit antibody; RFC36 antibody; RFC5 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of RFC5 recombinant protein (Accession Number: NM_007370). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

  • Validations

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:114626(RFC5 Antibody) at dilution of 1:1000

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:114626(RFC5 Antibody) at dilution of 1:1000

  • Background
    The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C(RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. Replication factor C5 (RFC5, synonyms: RFC36, MGC1155) is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA and form a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"