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Product Name
RECQL4 antibody
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Description
RECQL4 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human kidney tissue, human testis tissue. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 140-150 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
ATP dependent DNA helicase Q4 antibody; DNA helicase antibody; RecQ like type 4 antibody; RecQ protein like 4 antibody; RECQ4 antibody; RECQL4 antibody; RTS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RECQL4 recombinant protein (Accession Number: BC013277). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114676(RECQL4 antibody) at dilution of 1:1000
Immunofluorescent analysis of HepG2 cells, using RECQL4 antibody Catalog No:114676 at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-RECQL4 (IP:Catalog No:114676, 4ug; Detection:Catalog No:114676 1:1000) with HeLa cells lysate 2500ug.
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:114676(RECQL4 Antibody) at dilution of 1:50 (under 40x lens)
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Background
RECQ protein-like 4(RECOL4), which has helicase domains contain 7 consensus motifs in the middle, is a gene responsible for Werner syndorme and Bloom syndrome. It forms a stable complex with UBR1 adn UBR2, which has a DNA-stimulated ATPase activity. RECOL4 may involve in repairment of DNA damage by interaction with APE1 and FEN1, key participants in base excision repair. This is a rabbit polyclonal antibody raised against part of C-terminal of RECQ4 of human origin.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"