REC8 Polyclonal Antibody

Cat.#: 165999

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Product Information

  • Product Name
    REC8 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to REC8
  • Tested applications
    WB
  • Species reactivity
    Human, Rat
  • Alternative names
    REC8 antibody; HR21spB antibody; REC8L1 antibody; Rec8p antibody; REC8 meiotic recombination protein antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human REC8 (NP_005123.2).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - REC8 Polyclonal Antibody

    Western blot - REC8 Polyclonal Antibody

    Western blot analysis of extracts of SGC-7901 cells, using REC8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.

  • Background
    Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II (By similarity).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"