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Product Name
REC8 antibody
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Description
REC8 Rabbit Polyclonal antibody. Positive WB detected in human testis tissue, K-562 cells. Observed molecular weight by Western-blot: 85-95kd
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Tested applications
ELISA, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
Cohesin Rec8p antibody; HR21spB antibody; REC8 antibody; REC8 homolog (yeast) antibody; REC8L1 antibody; Rec8p antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of REC8 recombinant protein (Accession Number: NM_005132). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
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Validations
human testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:114673(REC8 antibody) at dilution of 1:1000
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Background
REC8, also named as Rec8p and REC8L1, belongs to the rad21 family. This cohesin protein is required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II. Rec8 is present in an unphosphorylated form early in meiotic prophase but is phosphorylated prior to meiosis I. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human REC8. This antibody can recognize the monomeric, non-phosphorylated form(63-70kd) of REC8 and other forms(80-100kd) which represent either posttranslationally modified forms or alternative transcript products (PMID: 12615909,19463812).
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References
- Erenpreisa J, Cragg MS, Salmina K, Hausmann M, Scherthan H. The role of meiotic cohesin REC8 in chromosome segregation in gamma irradiation-induced endopolyploid tumour cells. Experimental cell research. 315(15):2593-603. 2009.
- Rosa AM, Dabas N, Byrnes DM, Eller MS, Grichnik JM. Germ cell proteins in melanoma: prognosis, diagnosis, treatment, and theories on expression. Journal of skin cancer. 2012:621968. 2012.
- Litvinov IV, Cordeiro B, Huang Y. Ectopic expression of cancer-testis antigens in cutaneous T-cell lymphoma patients. Clinical cancer research : an official journal of the American Association for Cancer Research. 20(14):3799-808. 2014.
- Yuan YF, Zhai R, Liu XM, Khan HA, Zhen YH, Huo LJ. SUMO-1 plays crucial roles for spindle organization, chromosome congression, and chromosome segregation during mouse oocyte meiotic maturation. Molecular reproduction and development. 81(8):712-24. 2014.
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