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Product Name
RCVRN Polyclonal Antibody
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Description
Polyclonal antibody to RCVRN
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
RCVRN antibody; RCV1 antibody; recoverin antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human RCVRN (NP_002894.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100 -
Validations
Western blot - RCVRN Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using RCVRN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.
Immunohistochemistry - RCVRN Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat retina using RCVRN antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - RCVRN Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse retina using RCVRN antibody at dilution of 1:200 (40x lens).
Immunofluorescence - RCVRN Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using RCVRN antibody . Blue: DAPI for nuclear staining.
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Background
Seems to be implicated in the pathway from retinal rod guanylate cyclase to rhodopsin. May be involved in the inhibition of the phosphorylation of rhodopsin in a calcium-dependent manner. The calcium-bound recoverin prolongs the photoresponse.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"