rCRAMP (rat cathelin-related antimicrobial peptide, cathelicidin; animals)
Not For Human Use, Lab Use Only.
Cat.#: 301780
Optional Service:
TFA RemovalWhat's this?
Special Price 430.1 USD
Availability:
4 weeks
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Product Name
rCRAMP (rat cathelin-related antimicrobial peptide, cathelicidin; animals)
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Documents
Batch to batch variation of the purity
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Sequence Shortening
GLVRKGGEKFGEKLRKIGQKIKEFFQKLALEIEQ
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Sequence
Gly-Leu-Val-Arg-Lys-Gly-Gly-Glu-Lys-Phe-Gly-Glu-Lys-Leu-Arg-Lys-Ile-Gly-Gln-Lys-Ile-Lys-Glu-Phe-Phe-Gln-Lys-Leu-Ala-Leu-Glu-Ile-Glu-Gln
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Length (aa)
34
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Peptide Purity (HPLC)
97.7%
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Molecular Formula
C181H302N50O48
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Molecular Weight
3946.73
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CAS No.
376364-38-4
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Source
Synthetic
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Form
Powder
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Description
Activity against E. coli DH5a, E. coli ML-35p, P. aeruginosa PAO1, P. aeruginosaMR3007, MRSA ATCC 33591, S. aureus 93918 with MIC in the range of 0.25-2 uM. The MIC increased from 2.4 to 18 uM in the case of MRSA with an increase in NaCl concentration to 100 mM. rCRAMP (rat cathelin-related antimicrobial peptide, cathelicidin; animals) has Antibacterial activity. rCRAMP (rat cathelin-related antimicrobial peptide, cathelicidin; animals) was found in Norway rat, Rattus norvegicus.
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Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides
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About TFA salt
Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.
TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.
TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.
In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.
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Molar Concentration Calculator
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Dilution Calculator
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Percent Concentration Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Peptide Property
- Analysed Sequence:H-GLVRKGGEKFGEKLRKIGQKIKEFFQKLALEIEQ-OH
- Chemical Formula:C181H302N50O48
- Sequence length:34
- Extinction coefficient:0 M-1cm-1
- GRAVY:-0.68
- Mw average:3946.62
- Theoretical pI:10.41
- Data Source:Peptide Property Calculator
GRAVY = grand average of hydropathy
X: Hydrophobic uncharged residues, like F I L M V W A and P
X: Basic residues, like R K H
X: Acidic residues, like D E
X: Polar uncharged residues, like G S T C N Q and Y
Related Products / Services
• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
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• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"