-
Product Name
RCHY1 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to RCHY1
-
Tested applications
WB
-
Species reactivity
Human, Mouse
-
Alternative names
RCHY1 antibody; ARNIP antibody; CHIMP antibody; PIRH2 antibody; PRO1996 antibody; RNF199 antibody; ZCHY antibody; ZNF363 antibody; ring finger and CHY zinc finger domain containing 1 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human RCHY1 (NP_056251.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:200 - 1:1000
-
Validations
Western blot - RCHY1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using RCHY1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
-
Background
Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, P73, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53. Monoubiquitinates the translesion DNA polymerase POLH. Contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"