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Product Name
RBM3 antibody
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Description
RBM3 Rabbit Polyclonal antibody. Positive IP detected in MCF-7 cells. Positive WB detected in HeLa cells, A375 cells, HepG2 cells, MCF7 cells, Raji cells. Positive IHC detected in human testis tissue, human brain tissue, human colon cancer tissue, human kidney tissue, human lung tissue, human ovary tissue, human skin tissue, human spleen tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 17-20 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
IS1 RNPL antibody; Putative RNA binding protein 3 antibody; RBM3 antibody; RNA binding motif protein 3 antibody; RNPL antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RBM3 recombinant protein (Accession Number: NM_006743). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114610(RBM3 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human testis using Catalog No:114610(RBM3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:114610(RBM3 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-RBM3 (IP:Catalog No:114610, 4ug; Detection:Catalog No:114610 1:300) with MCF-7 cells lysate 3200ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:114610(RBM3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
RBM3, also named as RNPL, is a cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. It reduces the relative abundance of microRNAs, when overexpressed. RBM3 enhances phosphoryaltion of translation initiation factors and active polysome formation. It is up-regulated in human tumors. RBM3 is a potential proto-oncogenic proteins upon overexpression. (PMID: 19900510 ).
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References
- Chip S, Zelmer A, Ogunshola OO. The RNA-binding protein RBM3 is involved in hypothermia induced neuroprotection. Neurobiology of disease. 43(2):388-96. 2011.
- Peretti D, Bastide A, Radford H. RBM3 mediates structural plasticity and protective effects of cooling in neurodegeneration. Nature. 518(7538):236-9. 2015.
- Jackson TC, Manole MD, Kotermanski SE, Jackson EK, Clark RS, Kochanek PM. Cold stress protein RBM3 responds to temperature change in an ultra-sensitive manner in young neurons. Neuroscience. 305:268-78. 2015.
- Zhu X, Zelmer A, Kapfhammer JP, Wellmann S. Cold-inducible RBM3 inhibits PERK phosphorylation through cooperation with NF90 to protect cells from endoplasmic reticulum stress. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 30(2):624-34. 2016.
- Tong G, Endersfelder S, Rosenthal LM. Effects of moderate and deep hypothermia on RNA-binding proteins RBM3 and CIRP expressions in murine hippocampal brain slices. Brain research. 1504:74-84. 2013.
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