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Product Name
RBM24 antibody
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Description
RBM24 Rabbit Polyclonal antibody. Positive IHC detected in human skeletal muscle tissue. Positive WB detected in HeLa cells.
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
dJ259A10.1 antibody; FLJ26355 antibody; FLJ30829 antibody; FLJ37697 antibody; RBM24 antibody; RNA binding motif protein 24 antibody; RNA binding protein 24 antibody; RNPC6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RBM24 recombinant protein (Accession Number: NM_153020). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114606(RBM24 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:114606(RBM24 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:114606(RBM24 Antibody) at dilution of 1:50 (under 40x lens)
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Background
RNA-binding proteins have a pivotal role in post-transcriptionally regulating gene expression. During early development they exhibit temporally and spatially regulated expression pattern, and affect the proteome of a single cell by controlling alternative splicing, transport, localization, and stability of their target RNAs [PMID:22510371]. Their expression are tissue-specific, and their crucial influence on the protein set-up especially in muscle cells has become recognized recently [PMID:21982546]. RBM24 is one RNA-binding protein and plays a fundamental role in regulating cardiac gene expression, sarcomeric assembly, and cardiac contractility and may thus represent a novel pathway to cardiomyopathy [PMID:20338237].
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References
- Grifone R, Xie X, Bourgeois A, Saquet A, Duprez D, Shi DL. The RNA-binding protein Rbm24 is transiently expressed in myoblasts and is required for myogenic differentiation during vertebrate development. Mechanisms of development. 134:1-15. 2014.
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