-
Product Name
RBM17 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to RBM17
-
Tested applications
WB, IHC
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
RBM17 antibody; SPF45 antibody; splicing factor 45 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RBM17 (NP_116294.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:200 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - RBM17 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using RBM17 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - RBM17 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat lung using RBM17 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - RBM17 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using RBM17 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - RBM17 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human endometrium cancer using RBM17 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - RBM17 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using RBM17 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - RBM17 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using RBM17 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - RBM17 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse spleen using RBM17 antibody at dilution of 1:100 (40x lens).
-
Background
Splice factor that binds to the single-stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"