-
Product Name
RBM15B antibody
- Documents
-
Description
RBM15B Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, HEK-293 cells, mouse brain tissue. Observed molecular weight by Western-blot: 100-120 kDa,70 kDa
-
Tested applications
ELISA, WB, IP
-
Species reactivity
Human, Mouse, Rat; other species not tested.
-
Alternative names
HUMAGCGB antibody; HuOTT3 antibody; One twenty two protein 3 antibody; OTT3 antibody; RBM15B antibody; RNA binding motif protein 15B antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of RBM15B recombinant protein (Accession Number: BC001367). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114600(RBM15B antibody) at dilution of 1:2000
IP Result of anti-RBM15B (IP:Catalog No:114600, 4ug; Detection:Catalog No:114600 1:500) with HeLa cells lysate 2500ug.
-
Background
RBM15B, one members of the SPEN (Split-end) family of proteins, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components. Present polyclonal anti-RBM15B antibody was produced by immunizing animals with C-terminus of RBM15B, which homolog with a short form of RBM15B(563aa, ~70kDa), thus this antibody can recognize full length of RBM15B(~100kDa) and splice-one(70kDa)
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"