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Product Name
RB1CC1 antibody
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Description
RB1CC1 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, Jurkat cells, K-562 cells, MCF7 cells. Positive IF detected in HepG2 cells, MCF-7 cells. Positive IHC detected in human liver cancer tissue, human breast cancer tissue. Observed molecular weight by Western-blot: 200kd
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CC1 antibody; DRAGOU14 antibody; FIP200 antibody; KIAA0203 antibody; RB1 inducible coiled coil 1 antibody; RB1CC1 antibody; RBICC antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RB1CC1 recombinant protein (Accession Number: XM_011517643). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:114490(RB1CC1 antibody) at dilution of 1:1000
Immunofluorescent analysis of HepG2 cells, using RB1CC1 antibody Catalog No:114490 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-RB1CC1 (IP:Catalog No:114490, 5ug; Detection:Catalog No:114490 1:500) with HEK-293 cells lysate 2500ug.
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:114490(RB1CC1 Antibody) at dilution of 1:50 (under 40x lens)
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Background
RB1CC1, also named as RBICC, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1CC1.
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References
- Seglen PO, Luhr M, Mills IG, Sætre F, Szalai P, Engedal N. Macroautophagic cargo sequestration assays. Methods (San Diego, Calif.). 75:25-36. 2015.
- Yao J, Jia L, Khan N. Deletion of autophagy inducer RB1CC1 results in degeneration of the retinal pigment epithelium. Autophagy. 11(6):939-53. 2015.
- Liu F, Fang F, Yuan H. Suppression of autophagy by FIP200 deletion leads to osteopenia in mice through the inhibition of osteoblast terminal differentiation. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 28(11):2414-30. 2013.
- Karanasios E, Stapleton E, Manifava M, Ktistakis NT. Imaging autophagy. Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ...
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