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RB1 antibody

Cat.#: 114489

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Product Information

  • Product Name
    RB1 antibody
  • Documents
  • Description
    RB1 Rabbit Polyclonal antibody. Positive IP detected in A431 cells. Positive WB detected in Jurkat cells, A431 cells. Positive IHC detected in human prostate cancer tissue, human lung cancer tissue. Observed molecular weight by Western-blot: 110kd
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    OSRC antibody; p105 Rb antibody; pp110 antibody; pRb antibody; RB1 antibody; retinoblastoma 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of RB1 recombinant protein (Accession Number: NM_000321). Purification method: Protein A purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:1000-1:10000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:114489(RB1 antibody) at dilution of 1:3000

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:114489(RB1 antibody) at dilution of 1:3000

    IP Result of anti-RB1 (IP:Catalog No:114489, 3ug; Detection:Catalog No:114489 1:500) with A431 cells lysate 3000ug.

    IP Result of anti-RB1 (IP:Catalog No:114489, 3ug; Detection:Catalog No:114489 1:500) with A431 cells lysate 3000ug.

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:114489(RB1 antibody) at dilution of 1:200 (under 10x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:114489(RB1 antibody) at dilution of 1:200 (under 10x lens)

  • Background
    RB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. It is a key regulator of entry into cell division that acts as a tumor suppressor. RB1 acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. RB1 controls histone H4 'Lys-20' trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. This antibody is a rabbit polyclonal antibody raised against human RB1 fusion protein.
  • References
    • Zhang J, Guo H, Zhang H. Putative tumor suppressor miR-145 inhibits colon cancer cell growth by targeting oncogene Friend leukemia virus integration 1 gene. Cancer. 117(1):86-95. 2011.
    • Jung HM, Phillips BL, Chan EK. miR-375 activates p21 and suppresses telomerase activity by coordinately regulating HPV E6/E7, E6AP, CIP2A, and 14-3-3ζ. Molecular cancer. 13:80. 2014.
    • Wang G, Lunardi A, Zhang J. Zbtb7a suppresses prostate cancer through repression of a Sox9-dependent pathway for cellular senescence bypass and tumor invasion. Nature genetics. 45(7):739-46. 2013.
    • Liao X, Yang S, Shao Z. Effect of exogenous p16ink4a and hRb1 genes on cell cycle regulation of osteosarcoma cell. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. 25(6):679-82. 2005.
    • Brown DI, Lassègue B, Lee M. Poldip2 knockout results in perinatal lethality, reduced cellular growth and increased autophagy of mouse embryonic fibroblasts. PloS one. 9(5):e96657. 2014.
    • Hu Z, Yu L, Zhu D. Disruption of HPV16-E7 by CRISPR/Cas system induces apoptosis and growth inhibition in HPV16 positive human cervical cancer cells. BioMed research international. 2014:612823. 2014.
    • Ding W, Hu Z, Zhu D. Zinc finger nucleases targeting the human papillomavirus E7 oncogene induce E7 disruption and a transformed phenotype in HPV16/18-positive cervical cancer cells. Clinical cancer research : an official journal of the American Association for Cancer Research. 20(24):6495-503. 2014.
    • Hu Z, Ding W, Zhu D. TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy. The Journal of clinical investigation. 125(1):425-36. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"