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Product Name
RASSF6 antibody
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Description
RASSF6 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human ovary tumor tissue, human prostate cancer tissue. Positive WB detected in U-937 cells, COLO 320 cells, HeLa cells, HepG2 cells, PC-3 cells. Positive IP detected in HepG2 cells. Observed molecular weight by Western-blot: 43 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp686K23225 antibody; RASSF6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RASSF6 recombinant protein (Accession Number: NM_001270391). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
U-937 cells were subjected to SDS PAGE followed by western blot with Catalog No:114483(RASSF6 antibody) at dilution of 1:500
IP Result of anti-RASSF6 (IP:Catalog No:114483, 4ug; Detection:Catalog No:114483 1:500) with HepG2 cells lysate 2400ug.
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:114483(RASSF6 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of Hela cells, using RASSF6 antibody Catalog No:114483 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
RASSF6 represents Ras association (RalGDS/AF-6) domain family member 6
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References
- Hesson LB, Dunwell TL, Cooper WN. The novel RASSF6 and RASSF10 candidate tumour suppressor genes are frequently epigenetically inactivated in childhood leukaemias. Molecular cancer. 8:42. 2009.
- Wen Y, Wang Q, Zhou C. Decreased expression of RASSF6 is a novel independent prognostic marker of a worse outcome in gastric cancer patients after curative surgery. Annals of surgical oncology. 18(13):3858-67. 2011.
- Mezzanotte JJ, Hill V, Schmidt ML. RASSF6 exhibits promoter hypermethylation in metastatic melanoma and inhibits invasion in melanoma cells. Epigenetics. 9(11):1496-503. 2014.
- Ye HL, Li DD, Lin Q. Low RASSF6 expression in pancreatic ductal adenocarcinoma is associated with poor survival. World journal of gastroenterology. 21(21):6621-30. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"