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Product Name
RASGRF1 Polyclonal Antibody
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Description
Polyclonal antibody to RASGRF1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse
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Alternative names
RASGRF1 antibody; CDC25 antibody; CDC25L antibody; GNRP antibody; GRF1 antibody; GRF55 antibody; H-GRF55 antibody; PP13187 antibody; ras-GRF1 antibody; ras-specific guanine nucleotide-releasing factor 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human RASGRF1 (NP_001139120.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100 -
Validations
Western blot - RASGRF1 Polyclonal Antibody
Western blot analysis of extracts of mouse brain, using RASGRF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - RASGRF1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using RASGRF1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - RASGRF1 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using RASGRF1 antibody . Blue: DAPI for nuclear staining.
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Background
Promotes the exchange of Ras-bound GDP by GTP.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"