RARA Polyclonal Antibody

Cat.#: 160066

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Product Information

  • Product Name
    RARA Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to RARA
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    RARA antibody; NR1B1 antibody; RAR antibody; retinoic acid receptor alpha antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-457 of human RARA (NP_001019980.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:100
    IF 1:50 - 1:100
  • Validations

    Western blot - RARA Polyclonal Antibody

    Western blot - RARA Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using RARA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.

    Immunohistochemistry - RARA Polyclonal Antibody

    Immunohistochemistry - RARA Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded rat lung using RARA antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - RARA Polyclonal Antibody

    Immunohistochemistry - RARA Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded rat brain using RARA antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - RARA Polyclonal Antibody

    Immunohistochemistry - RARA Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded mouse brain using RARA antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - RARA Polyclonal Antibody

    Immunohistochemistry - RARA Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded mouse cerebellum using RARA antibody at dilution of 1:100 (40x lens).

  • Background
    Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"