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Product Name
RANKL antibody
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Description
RANKL Rabbit Polyclonal antibody. Positive IHC detected in human colon tissue. Positive IP detected in COLO 320 cells. Positive WB detected in Transfected HEK-293 cells.
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
CD254 antibody; hRANKL2 antibody; ODF antibody; OPGL antibody; OPTB2 antibody; Osteoprotegerin ligand antibody; RANKL antibody; sOdf antibody; TNFSF11 antibody; TRANCE antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RANKL recombinant protein (Accession Number: NM_003701). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:114544(RANKL Antibody) at dilution of 1:700
IP Result of anti-RANKL (IP:Catalog No:114544, 4ug; Detection:Catalog No:114544 1:300) with COLO 320 cells lysate 2000ug.
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:114544(RANKL Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:114544(RANKL Antibody) at dilution of 1:50 (under 40x lens)
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Background
TNFSF11 represents tumor necrosis factor (ligand) superfamily, member 11
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