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Product Name
RANGAP1 Polyclonal Antibody
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Description
Polyclonal antibody to RANGAP1
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Tested applications
WB, IF
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Species reactivity
Human, Duck
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Alternative names
RANGAP1 antibody; Fug1 antibody; RANGAP antibody; SD antibody; ran GTPase-activating protein 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 398-587 of human RANGAP1 (NP_002874.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - RANGAP1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using RANGAP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - RANGAP1 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using RANGAP1 antibody .
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Background
GTPase activator for RAN. Converts cytoplasmic GTP-bound RAN to GDP-bound RAN, which is essential for RAN-mediated nuclear import and export. Mediates dissociation of cargo from nuclear export complexes containing XPO1, RAN and RANBP2 after nuclear export.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"