Ranatuerin-2Pb peptide

Ranatuerin-2Pb peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-SFLTTVKKLVTNLAALAGTVIDTIKCKVTGGCRT-OH

H-Ser-Phe-Leu-Thr-Thr-Val-Lys-Lys-Leu-Val-Thr-Asn-Leu-Ala-Ala-Leu-Ala-Gly-Thr-Val-Ile-Asp-Thr-Ile-Lys-Cys-Lys-Val-Thr-Gly-Gly-Cys-Arg-Thr-OH

34

95.2%

C₁₅₅H₂₇₂N₄₂O₄₆S₂

3524.18

Synthetic

Powder

Ranatuerin-2Pb is an antimicrobial peptide identified from Frog Skin Secretion of Rana pipiens. Ranatuerin-2Pb exhibited broad-spectrum antimicrobial activity. Ranatuerin-2Pb exhibited strong antimicrobial activity against S. aureus, E. coli, C. albicans and MRSA; however, it showed no inhibition activity against E. faecalis and P. aeruginosa. Ranatuerin-2Pb exhibited a hemolysis rate near 20% on horse erythrocytes with the concentration at 8uM. The HC50 of ranatuerin-2Pb was 16.11 uM. Ranatuerin-2Pb showed antibiofilm activity against S. aureus, E. coli and C. albicans. Ranatuerin-2Pb exhibited significant inhibitory effect on the proliferation of NCI-H157, MCF-7, U251MG and PC-3, with the IC50 value of 1.453 µM, 7.254 µM, 2.172 µM and 2.251 µM, respectively. Ranatuerin-2Pb showed no inhibitory effect on the proliferation of MDA-MB-435s. Ranatuerin-2Pb induced the highest degree of LDH release for H157 among three peptides.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Zhou X, Shi D, Zhong R, et al. Bioevaluation of Ranatuerin-2Pb from the Frog Skin Secretion of Rana pipiens and its Truncated Analogues. Biomolecules. 2019;9(6)

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.