RANATUERIN 2P peptide

RANATUERIN 2P peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

GLMDTVKNVAKNLAGHMLDKLKCKITGC

Gly-Leu-Met-Asp-Thr-Val-Lys-Asn-Val-Ala-Lys-Asn-Leu-Ala-Gly-His-Met-Leu-Asp-Lys-Leu-Lys-Cys-Lys-Ile-Thr-Gly-Cys

28

95.8%

Synthetic

Powder

Disulfide bridge: C22,C27. Active against S. aureus (MIC 50 uM), E. coli (MIC 13 uM), and C. albicans (MIC 67 uM). This peptide could inhibit HIV infection at a concentration that is also toxic to T cells (J Virol 2005; 79:11598-11606). Found in multiple species. Updated 3/2015. The source of RANATUERIN 2P is North American frog, Rana pipiens , ALSO: the Oregon spotted frog Rana pretiosa.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Goraya J, Wang Y, Li Z, et al. Peptides with antimicrobial activity from four different families isolated from the skins of the North American frogs Rana luteiventris, Rana berlandieri and Rana pipiens. Eur J Biochem. 2000;267(3):894-900.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.