Ranalexin peptide

Ranalexin peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

FLGGLIKIVPAMICAVTKKC

H-Phe-Leu-Gly-Gly-Leu-Ile-Lys-Ile-Val-Pro-Ala-Met-Ile-Cys-Ala-Val-Thr-Lys-Lys-Cys-OH (Disulfide bond)

20

97.1%

C₉₇H₁₆₇N₂₃O₂₂S₃

2103.7

Synthetic

Powder

Recombinant peptide is as active as the WT against S. aureus, S. pyogenes, E. coli, and MRSA (MIC 8 and 128 ug/ml). The recombinant ranalexin is also cytotoxic in HeLa and COS7 human cancer cells (IC50 =13-15 ug/ml)(Appl Microbiol Biotechnol. 2012). In membrane-mimetic TFE or DPC micelles, residues 8-15 are helical (edited 11/4/2007 GW). In an animal model:mouse for systemic infection, the peptide showed synergistic effect against MRSA with antistaphylococcal endopeptidase (Desbois AP et al., 2010 Int J Antimicrob Agents 35: 559-65). Updated 10/12/2012 GW. Ranalexin has Antibacterial, Antifungal activity. Ranalexin was found in Bull frog, Rana catesbeiana, North America.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.