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Product Name
RAMP2 antibody
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Description
RAMP2 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, SH-SY5Y cells. Positive IHC detected in human breast cancer. Observed molecular weight by Western-blot: 20kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
RAMP2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RAMP2 recombinant protein (Accession Number: NM_005854). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114534(RAMP2 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114534(RAMP2 antibody) at dilution of 1:100 (under 10x lens)
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Background
RAMP2 (receptor-activity-modifying protein) is a member of the RAMP family of single-transmembrane-domain proteins which consist of an N-terminal extracellular domain, a transmembrane region and a short intracellular C-terminal tail. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a G protein-coupled receptor, can function as either a calcitonin gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. RAMP1 transports the CRLR to the plasma membrane and then remains associated with it to function as a terminally glycosylated CGRP receptor, while RAMP2 and RAMP3 transfer the CRLR to the cell surface to generate receptors that are preferentially selective for adrenomedullin. (PMID: 9620797; 16188935)
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References
- MacManus CF, Campbell EL, Keely S, Burgess A, Kominsky DJ, Colgan SP. Anti-inflammatory actions of adrenomedullin through fine tuning of HIF stabilization. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 25(6):1856-64. 2011.
- Hayashi KG, Hosoe M, Sakumoto R, Takahashi T. Temporo-spatial expression of adrenomedullin and its receptors in the bovine placenta. Reproductive biology and endocrinology : RB&E. 11:62. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"