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Product Name
RAD18 Polyclonal Antibody
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Description
Polyclonal antibody to RAD18
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
RAD18 antibody; RNF73 antibody; E3 ubiquitin-protein ligase RAD18 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 216-495 of human RAD18 (NP_064550.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - RAD18 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using RAD18 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 120s.
Immunofluorescence - RAD18 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using RAD18 antibody . Blue: DAPI for nuclear staining.
Immunofluorescence - RAD18 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using RAD18 antibody . Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.
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Background
E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"