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RAD18 antibody

Cat.#: 114511

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Special Price 239.0 USD

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Product Information

  • Product Name
    RAD18 antibody
  • Documents
  • Description
    RAD18 Rabbit Polyclonal antibody. Positive WB detected in K-562 cells, A549 cells, HeLa cells, MCF7 cells. Observed molecular weight by Western-blot: 75kd,85kd
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    RAD18 antibody; RAD18 homolog (S. cerevisiae) antibody; hHR18 antibody; hRAD18 antibody; RNF73 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of RAD18 recombinant protein (Accession Number: NM_020165). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

  • Validations

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:114511(RAD18 antibody) at dilution of 1:300

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:114511(RAD18 antibody) at dilution of 1:300

  • Background
    RAD18(E3 ubiquitin-protein ligase RAD18) is lalso named as RNF73, hHR18 and belongs to the RAD18 family. It is involved in postreplication repair of UV-damaged DNA. RAD18 protein is detected in human cells as two major bands at 75 and 85 kDa by Western blot. The bands are identified as nonubiquitinated and monoubiquitinated forms of Rad18, respectively. Monoubiquitinated RAD18 is detected mainly in the cytoplasm, whereas nonubiquitinated RAD18 is detected predominantly in the nuclei(PMID:15509568). This antibody is specific to RAD18.
  • References
    • Sharma S, Hicks JK, Chute CL. REV1 and polymerase ζ facilitate homologous recombination repair. Nucleic acids research. 40(2):682-91. 2012.
    • Sharma S, Shah NA, Joiner AM, Roberts KH, Canman CE. DNA polymerase ζ is a major determinant of resistance to platinum-based chemotherapeutic agents. Molecular pharmacology. 81(6):778-87. 2012.
    • Liu C, Wang D, Wu J, Keller J, Ma T, Yu X. RNF168 forms a functional complex with RAD6 during the DNA damage response. Journal of cell science. 126(Pt 9):2042-51. 2013.
    • Lu LY, Xiong Y, Kuang H, Korakavi G, Yu X. Regulation of the DNA damage response on male meiotic sex chromosomes. Nature communications. 4:2105. 2013.
    • Helchowski CM, Skow LF, Roberts KH, Chute CL, Canman CE. A small ubiquitin binding domain inhibits ubiquitin-dependent protein recruitment to DNA repair foci. Cell cycle (Georgetown, Tex.). 12(24):3749-58. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"