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Product Name
Rabbit anti HA-Tag pAb
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Description
Polyclonal antibody to Rabbit anti HA-Tag
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Tested applications
WB, IF, ChIP
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Alternative names
antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of YPYDVPDYA (HA-tag)-KLH
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IF 1:50 - 1:100
ChIP 1:20 - 1:50 -
Validations
Western blot - Rabbit anti HA-Tag pAb
Western blot analysis of over-expressed HA-tagged protein in 293T cell using HA-tag antibody at different dilution. Each lane was loaded with 2 ug cell lysate.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Blocking buffer: 3% nonfat dry milk in TBST.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"