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Product Name
Rabbit anti GST-Tag pAb
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Description
Polyclonal antibody to Rabbit anti GST-Tag
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Tested applications
WB, IF, IP
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human GST-Tag
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:1000 - 1:2000
IF 1:20 - 1:50
IP 1:20 - 1:50 -
Validations
Western blot - Rabbit anti GST-Tag pAb
Western blot analysis of GST-tagged protein using GST-tag antibody at different dilution. Each lane was loaded with 5 ug protein.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - Rabbit anti GST-Tag pAb
Western blot analysis of over-expressed GST fusion protein using GST-tag antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"