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Product Name
Rabbit anti GFP-Tag pAb
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Description
Polyclonal antibody to Rabbit anti GFP-Tag
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Tested applications
WB, IHC, IF
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Alternative names
antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of GFP
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:2000 - 1:3000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - Rabbit anti GFP-Tag pAb
Western blot analysis of extracts of normal 293T cells and 293T transfected with EGFP, using GFP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunofluorescence - Rabbit anti GFP-Tag pAb
Immunofluorescence analysis of GFP transgenic 293T cells using GFP-Tag antibody . Green: GFP expression. Blue: DAPI for nuclear staining.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"