-
Product Name
Rabbit anti DDDDK-Tag mAb
- Documents
-
Description
Monoclonal antibody to Rabbit anti DDDDK-Tag
-
Tested applications
WB, IHC, IF, IP
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthetic Peptide of human DDDDK-Tag
-
Clonality
Monoclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:2000 - 1:5000
IHC 1:50 - 1:500
IF 1:50 - 1:500
IP 1:50 - 1:500 -
Validations
Western blot - Rabbit anti DDDDK-Tag mAb
Western blot analysis of extracts of over-expressed DDDDK-tagged protein in 293T cells, using Rabbit anti DDDDK-Tag mAb at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunofluorescence - Rabbit anti DDDDK-Tag mAb
Immunofluorescence analysis of GFP-DDDDK transgenic HeLa cells using DDDDK-Tag antibody . Green: GFP expression. Blue: DAPI for nuclear staining.
Immunoprecipitation - Rabbit anti DDDDK-Tag mAb
Immunoprecipitation of over-expressed DDDDK-tagged protein in 293T cells incubated using DDDDK-tag antibody . A mock served as negative control and over-expressed 293T cell lysate served as positive control.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"