Rabbit anti DDDDK-Tag mAb

Cat.#: 166307

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Product Information

  • Product Name
    Rabbit anti DDDDK-Tag mAb
  • Documents
  • Description
    Monoclonal antibody to Rabbit anti DDDDK-Tag
  • Tested applications
    WB, IHC, IF, IP
  • Alternative names
    antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic Peptide of human DDDDK-Tag
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:2000 - 1:5000
    IHC 1:50 - 1:500
    IF 1:50 - 1:500
    IP 1:50 - 1:500
  • Validations

    Western blot - Rabbit anti DDDDK-Tag mAb

    Western blot - Rabbit anti DDDDK-Tag mAb

    Western blot analysis of extracts of over-expressed DDDDK-tagged protein in 293T cells, using Rabbit anti DDDDK-Tag mAb at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.

    Immunofluorescence - Rabbit anti DDDDK-Tag mAb

    Immunofluorescence - Rabbit anti DDDDK-Tag mAb

    Immunofluorescence analysis of GFP-DDDDK transgenic HeLa cells using DDDDK-Tag antibody . Green: GFP expression. Blue: DAPI for nuclear staining.

    Immunoprecipitation - Rabbit anti DDDDK-Tag mAb

    Immunoprecipitation - Rabbit anti DDDDK-Tag mAb

    Immunoprecipitation of over-expressed DDDDK-tagged protein in 293T cells incubated using DDDDK-tag antibody . A mock served as negative control and over-expressed 293T cell lysate served as positive control.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"