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Product Name
RAB5A-Specific antibody
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Description
RAB5A-Specific Rabbit Polyclonal antibody. Positive IHC detected in human brain tissue, human placenta tissue. Positive IF detected in NIH/3T3 cells. Positive WB detected in human brain tissue, mouse colon tissue, SH-SY5Y cells. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 24 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
RAB5 antibody; RAB5A antibody; Ras related protein Rab 5A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_004162). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114454(RAB5A-Specific antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:114454(RAB5A-Specific Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:114454(RAB5A-Specific Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of (-20oc Ethanol) fixed NIH/3T3 cells using Catalog No:114454(RAB5A-Specific Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
IP Result of anti-RAB5A-Specific (IP:Catalog No:114454, 4ug; Detection:Catalog No:114454 1:600) with mouse brain tissue lysate 4000ug.
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Background
RAB5A belongs to the small GTPase superfamily and Rab family. It is required for the fusion of plasma membranes and early endosomes. The antibody is specific to RAB5A. It has no cross reaction to RAB5B or RAB5C.
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