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Product Name
RAB5A antibody
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Description
RAB5A Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, HeLa cells, human brain tissue, rat brain tissue. Observed molecular weight by Western-blot: 24 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
RAB5 antibody; RAB5A antibody; Ras related protein Rab 5A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RAB5A recombinant protein (Accession Number: NM_004162). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114453(RAB5A antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:114453(RAB5A antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-RAB5A (IP:Catalog No:114453, 4ug; Detection:Catalog No:114453 1:500) with mouse brain tissue lysate 4000ug.
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Background
RAB5A represents RAB5A, member RAS oncogene family
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References
- Wu KY, He M, Hou QQ. Semaphorin 3A activates the guanosine triphosphatase Rab5 to promote growth cone collapse and organize callosal axon projections. Science signaling. 7(340):ra81. 2014.
- Pan Y, Wang R, Zhang F. MicroRNA-130a inhibits cell proliferation, invasion and migration in human breast cancer by targeting the RAB5A. International journal of clinical and experimental pathology. 8(1):384-93. 2015.
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