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Product Name
RAB21 Polyclonal Antibody
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Description
Polyclonal antibody to RAB21
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
RAB21 antibody; ras-related protein Rab-21 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human RAB21 (NP_055814.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - RAB21 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using RAB21 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunofluorescence - RAB21 Polyclonal Antibody
Immunofluorescence analysis of C6 cells using RAB21 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - RAB21 Polyclonal Antibody
Immunofluorescence analysis of NIH-3T3 cells using RAB21 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - RAB21 Polyclonal Antibody
Immunofluorescence analysis of U-2 OS cells using RAB21 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - RAB21 Polyclonal Antibody
Immunofluorescence analysis of U-2 OS cells using RAB21 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Regulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general (By similarity). As a result, may regulate cell adhesion and migration (By similarity). During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis. Involved in neurite growth (By similarity). Following SBF2/MTMT13-mediated activation in response to starvation-induced autophagy, binds to and regulates SNARE protein VAMP8 endolysosomal transport required for SNARE-mediated autophagosome-lysosome fusion.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"