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Product Name
RAB20 antibody
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Description
RAB20 Rabbit Polyclonal antibody. Positive IP detected in SW 1990 cells. Positive WB detected in SW 1990 cells, HeLa cells. Observed molecular weight by Western-blot: 26-29 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ20429 antibody; RAB20 antibody; Ras related protein Rab 20 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RAB20 recombinant protein (Accession Number: NM_017817). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
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Validations
SW 1990 cells were subjected to SDS PAGE followed by western blot with Catalog No:114419(RAB20 antibody) at dilution of 1:1000
IP Result of anti-RAB20 (IP:Catalog No:114419, 4ug; Detection:Catalog No:114419 1:1000) with SW 1990 cells lysate 2400ug.
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Background
RAB20 is a member of RAS oncogene family, it has no close relationship to any other members of RAB subfamily which consists of small GTP-binding proteins involved in the regulation of intracellullar vesicular transport. Firstly found highly expressed on the apical side of kidney tubuel epithelial cells, RAB20 was speculated to be involved in apical endocytosi and recylcing processes. So far, its function is not well known, however, RAB20 is recently found higher expressed in pancreatic adenocarcinomas than normal pancreas, which is proved by WB and IHC analyses, suggesting its promising role in diagnosis of pancreatic carcinogenesis. Catalog#11616-1-AP is a rabbit polyclonal antibody raised against full-length of human RAB20.
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References
- Pei G, Repnik U, Griffiths G, Gutierrez MG. Identification of an immune-regulated phagosomal Rab cascade in macrophages. Journal of cell science. 127(Pt 9):2071-82. 2014.
- Pei G, Schnettger L, Bronietzki M, Repnik U, Griffiths G, Gutierrez MG. Interferon-γ-inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages. Molecular biology of the cell. 26(17):3061-70. 2015.
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