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Product Name
Rab18 antibody
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Description
Rab18 Rabbit Polyclonal antibody. Positive IP detected in mouse testis tissue. Positive WB detected in human testis tissue, human brain tissue, human heart tissue, mouse testis tissue, PC-3 cells. Positive IHC detected in human prostate cancer tissue, human breast cancer tissue. Observed molecular weight by Western-blot: 20-23 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
RAB18 antibody; RAB18LI1 antibody; Ras related protein Rab 18 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Rab18 recombinant protein (Accession Number: NM_021252). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
human testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:114415(RAB18 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:114415(RAB18 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:114415(RAB18 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-RAB18 (IP:Catalog No:114415, 3ug; Detection:Catalog No:114415 1:300) with mouse testis tissue lysate 4000ug.
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Background
RAB18 is a member of a family of Ras-related small GTPases. RAB18 plays a role in apical endocytosis/recycling, and may be implicated in transport between the plasma membrane and early endosomes. It also plays a key role in eye and brain development and neurodegeneration. Defects in RAB18 are associated with Warburg micro syndrome type 3.
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References
- You X, Liu F, Zhang T, Li Y, Ye L, Zhang X. Hepatitis B virus X protein upregulates oncogene Rab18 to result in the dysregulation of lipogenesis and proliferation of hepatoma cells. Carcinogenesis. 34(7):1644-52. 2013.
- Zhong K, Chen K, Han L, Li B. MicroRNA-30b/c inhibits non-small cell lung cancer cell proliferation by targeting Rab18. BMC cancer. 14:703. 2014.
- Cheng CY, Wu JC, Tsai JW. ENU mutagenesis identifies mice modeling Warburg Micro Syndrome with sensory axon degeneration caused by a deletion in Rab18. Experimental neurology. 267:143-51. 2015.
- Salloum S, Wang H, Ferguson C, Parton RG, Tai AW. Rab18 binds to hepatitis C virus NS5A and promotes interaction between sites of viral replication and lipid droplets. PLoS pathogens. 9(8):e1003513. 2013.
- Gerondopoulos A, Bastos RN, Yoshimura S. Rab18 and a Rab18 GEF complex are required for normal ER structure. The Journal of cell biology. 205(5):707-20. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"