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Product Name
RAB11-Specific antibody
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Description
RAB11-Specific Rabbit Polyclonal antibody. Positive IP detected in SH-SY5Y cells. Positive WB detected in human brain tissue, mouse colon tissue, SH-SY5Y cells. Positive IF detected in IMCD3 cells. Observed molecular weight by Western-blot: 24 kDa, 29 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Rab 11 antibody; RAB11 antibody; RAB11A antibody; Ras related protein Rab 11A antibody; YL8 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_004663). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114411(RAB11A-Specific antibody) at dilution of 1:500
IF result of Catalog No:114411 (anti-RAB11A,Green) in ciliated IMCD3 cells by Dr. Seongjin Seo. (Red is the primary cilium, and blue is the nucleus)
IP Result of anti-RAB11A-Specific (IP:Catalog No:114411, 3ug; Detection:Catalog No:114411 1:700) with SH-SY5Y cells lysate 3000ug.
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Background
Rab11a, Rab11b and Rab25 comprise a subfamily of Rab proteins that belong to the Ras-related small GTPase protein superfamily (PMID: 8074219, PMID: 10708602). Rab11 proteins are involved in endocytosis and plasma membrane recycling in non-polarized and polarized cells ( PMID: 8638719, PMID: 8922376). Rab11a and Rab11b are ubiquitously expressed in most tissues, while Rab25 is only expressed in epithelial cells. Rab11a is known to regulate the recycling of internalized cell surface proteins and receptors from the early endosome through the trans-Golgi network (PMID: 11121436, PMID: 9600939). The antibody is specific to RAB11A, and has no cross reaction to RAB11B.
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References
- Humbert MC, Weihbrecht K, Searby CC. ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting. Proceedings of the National Academy of Sciences of the United States of America. 109(48):19691-6. 2012.
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