Human RSPO3 (aa 1-146, His Tag) recombinant protein

Human RSPO3 (aa 1-146, His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway.

R-spondin-3

R-SPONDIN 3; PWTSR; FLJ14440; RSPO3; CRISTIN1; THSD2; AW742308; MGC118442; 2810459H04RIK

Q9BXY4

RSPO3; PWTSR; THSD2

Human Cells

A DNA sequence encoding the amino acids (Met 1-Val 146) of human RSPO3 (Q9BXY4-1) was fused with a polyhistidine tag at the C-terminus.

Human

The recombinant human RSPO3 consists of 136 amino acids and predictes a molecular mass of 15.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh RSPO3 is approximately 22-27 kDa due to different glycosylation.

> 96 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA.
2. Immobilized RSPO3-His (146) at 10 μg/mL (100 μL/well) can bind human RNF43-Fc (Cat:503014).
The EC₅₀ of human RNF43-Fc is 0.01-0.03μg/mL.